biotin cd19 (clone 1d3)  (Thermo Fisher)

Journal: iScience

Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

doi: 10.1016/j.isci.2025.112800

Figure Lengend Snippet: IRF4 deficiency compromises MHC class Ⅱ expression and further restrains ILC-mediated apoptosis of effector CD4 + T cells both in vitro and in vivo (A) Violin plots visualizing the expression of MHC-class-Ⅱ-related signature genes. (B) FACS analysis and MFI of MHC class Ⅱ expression in ILC3s isolated from Irf4 f/f and Irf4 f/f Rorc cre mice ( n = 6). ILC3 subsets were gated as Lin − RORγt + and then CCR6 + NKp46 − , CCR6 − NKp46 + , or CCR6 − NKp46 − . The lineage cocktail included TCRγδ, CD3ε, CD19, CD5, CD11c, Gr-1, and Ter119. (C and D) Activated OT-Ⅱ CD4 + T cells were cultured ex vivo with purified ILC3s from the siLP of Irf4 f/f and Irf4 f/f Rorc cre mice in the presence or absence of Ova peptide or the anti-MHC class Ⅱ neutralizing antibody. (C) Quantification of OT-Ⅱ T cells recovery (%). (D) Quantification of Annexin-V + OT-Ⅱ T cells. (E and F) Naive CD4-positive T cells (gating as CD4 + CD25 − CD62L hi CD44 lo cells) were sorted from OT-Ⅱ mice and pre-activated overnight. After pre-activation, CD4 positive T cells were transplanted into recipient Irf4 f/f and Irf4 f/f Rorc cre mice along with OVA peptide administration every 2 days following transfer. Nine days later, mice were sacrificed for further analysis of OT-Ⅱ CD4 + T cells (gating as CD8 − CD4 + TCRβ + Vβ5 + ) transferred in the spleen, mLN, siLPL, and cLPL of recipient mice. (G) One hundred thousand intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) were sorted from Irf4 f/f or Irf4 f/f Rorc cre mice and transferred with 500,000 activated OT-ⅡCD4 + T cells into NCG mice, flowing by OVA peptide i.p. every 2 days. Nine days later, survived OT-ⅡCD4 + T cells were quantified. ( n = 2 APC, n = 5 transferred group). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments (B–G). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

Article Snippet: Biotin CD19 (clone 1D3) , eBioscience , Cat# 13-0193-86; RRID: AB_657655.

Techniques: Expressing, In Vitro, In Vivo, Isolation, Cell Culture, Ex Vivo, Purification, Activation Assay, Two Tailed Test

Journal: iScience

Article Title: Canonical IRE1 function needed to sustain vigorous natural killer cell proliferation during viral infection

doi: 10.1016/j.isci.2023.108570

Figure Lengend Snippet:

Article Snippet: Rat anti-mouse CD19 (clone 1D3), biotin conjugated , Thermo Fisher Scientific , Cat# 13-0193-85; RRID: AB_657658.

Techniques: Blocking Assay, Virus, Recombinant, Cell Culture, Binding Assay, Isolation, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Plasmid Preparation, Software

Journal: eLife

Article Title: A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment

doi: 10.7554/eLife.37851

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-mouse CD19 Biotin (clone 1D3/6D5) , eBioscience , Cat# 13-0193-85; RRID: AB_657658 , (1:100).

Techniques: Recombinant, Plasmid Preparation, Generated, Derivative Assay, Control, Expressing, Modification, Software, Transfection